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Banana DNA
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== Demonstration == Preparation: prepare the extraction solution. For every 500mL of liquid soap, add: * 146g Salt * 73g Sodium Citrate * 5g EDTA This demonstration requires about 15mL of the extraction solution for each run, so adjust accordingly for the number of times you expect to present this demonstration. If you'd like, you can pre-mash the fruit for the demonstration, but it is better to mash it as needed. # Give each group of 2-4 students two of the centrifuge tubes, a skewer, and a small wad of gauze. Inform them that they will be extracting DNA from the fruit you brought in. # Mash up the fruit, and give each student a small scoop (about 10mL) in one of their centrifuge tubes. Have them add 15mL of the extraction solution, then put the cap on and shake the tube for 1-2 minutes. # Have the students now put the gauze over the second centrifuge tube, and slowly pour the solution through the gauze to remove any big chunks. # Add between 10 and 20mL of the chilled ethanol into their strained solution, and have them gently swirl it. They will see long strands start to precipitate out! Have them use their skewer to collect the strands and lift them out of the solution. Those strands are the DNA from the fruit!
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